Recent advances in wheat transformation |
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Authors: | Vasant janakiraman Martin Steinau Serena B McCoy Harold N Trick |
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Institution: | (1) Department of Plant Pathology, Kansas State University, 66506-5502 Manhattan, KS |
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Abstract: | Summary Since the first report of wheat transformation in the early 1990s, genetic engineering of wheat has evolved rapidly. Several
laboratories worldwide have reported the production of fertile transgenic wheat plants using a variety of methods. While there
are several innovative and promising approaches for wheat transformation using different explants as targets for transformation,
different methods of transformation, and different selection schemes, the most common approach to wheat transformation is
the bombardment of tissue derived from immature embryos followed by selection based on resistance to the bar gene. Even with all these successful reports, hurdles still exist for this recalcitrant crop. Of these hurdles, low transformation
rates, tools for transgene expression, and transgene silencing in subsequent generations are probably the most critical. This
review will provide an overview of wheat transformation in the past decade, addressing both positive and negative factors
that effect transformation while highlighting the successes of the past and prospects for the future. |
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Keywords: | Triticum aestivum genetic engineering review tissue culture |
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