Regeneration of zoysia grass (Zoysia matrella L. Merr.) cv. Konhee from young inflorescences and stem nodes |
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Authors: | Murugesan Dhandapani Seung-Beom Hong Channa Reddy Aswath Doo Hwan Kim |
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Institution: | (1) Department of Molecular Biotechnology, Konkuk University, Hwayang-dong, Kwangjin-gu, 143-701 Seoul, South Korea;(2) Indian Institute of Horticultural Research, 560089 Bangalore, India |
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Abstract: | We have optimized conditions for efficient regeneration of the vegetatively propagated zoysia grass (Zoysia matrella L. Merr) cultivar “Konhee”. Two explants, young inflorescences, and stem nodes, were used and they displayed different responses
to combinations and concentrations of plant growth regulators in callusing, embryogenic callus formation, and regeneration.
The highest callus initiation rate from young inflorescences was obtained on medium supplemented with 4.5 to 9.0 μM 2,4-dicholorophenoxy
acetic acid (2,4-D) and 0.44 μM 6-benzyl amino purine (BA). When the BA concentration was lowered to 0.044 μM, the highest
percent embryogenic callus induction from young inflorescences was achieved. The highest callus initiation rate from stem
nodes was obtained, when young inflorescences were cultured on MS medium supplemented with 4.5 to 9.0 μM 2,4-D, 0.44 μM BA,
and 0.037 μM abscisic acid (ABA). But embryogenic callus formation from the stem node was highest in the presence of 4.5 to
9.0 μM 2,4-D, 0.044 μM BA, and 0.037 μM ABA. Addition of ABA significantly increased embryogenic callus formation from stem
nodes, but not from young inflorescences. Regeneration percentage was variable in response to BA level, and inclusion of α-naphthalene
acetic acid (NAA) and gibberellic acid (GA3) further increased the regeneration percentage. The highest regeneration percentages obtained from the young inflorescences
and stem nodes were 82% and 67%, respectively. This is the first report showing that plants can be regenerated from young
inflorescences and stem nodes of vegetatively propagated zoysia grass. |
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Keywords: | Zoysia matrella Konhee Young inflorescences Stem node Embryogenic callus Plant regeneration |
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