The development of an efficient cultivar-independent plant regeneration system from callus derived from both apical and non-apical root segments of garlic (<Emphasis Type="Italic">Allium sativum</Emphasis> L.) |
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Authors: | Si-Jun?Zheng Betty?Henken Frans?A?Krens Email author" target="_blank">Chris?KikEmail author |
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Institution: | (1) Plant Research International, Wageningen University and Research Center, P.O. Box 16, 6700 AA Wageningen, The Netherlands |
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Abstract: | Summary Callus induction and later plant regeneration were studied in four widely grown garlic (Allium sativum L.) cultivars from Europe. Root segments from in vitro plantlets were used as starting material. In addition to cultivar effects, the effects of auxin and cytokinin levels and
the position of the segments on the root were studied. There were no statistically significant differences among cultivars
for the number of root segments that induced callus in the two series of experiments. The average induction frequency was
34.7% in the first series of experiments. Callus induction on apical root segments was significantly higher compared to callus
induction on non-apical root segments in the second series of experiments. Two months after callus induction, callus lines
were transferred to a regeneration medium consisting of Murashige and Skoog basal medium supplemented with 30gl−1 sucrose and 1 mgl−1 (4.6μM) kinetin. Calluses derived from different experiments were quite uniform with respect to their regeneration potential. Also
it was found that our regeneration system was cultivar-independent. The average shoot regeneration frequency was 17.9% in
the first series of experiments. Highly significant differences were found in the frequency of shoot regeneration among different
callus induction treatments. When the cytokinin 6-(γ,γ-dimethylallylamino)purine (0.1mgl−1∶0.5 μM) was present during callus induction, shoot regeneration ranged from 30.10 to 47.60%. Shoot regeneration from callus induced
on non-apical segments was higher, although not significant, compared to callus induction from apical root segments in the
second series of experiments. All in all, an efficient callus induction and plant regeneration system was developed from both
apical and non-apical segments taken along the entire length of the roots. This system has potential to be used for garlic
transformation. |
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Keywords: | callus induction in vitro plantlets root system shoot regeneration |
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