A further study on the regulation of cyclic nucleotide phosphodiesterase activity in neuroblastoma cells: Effect of growth |
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Authors: | Pramod K Sinha Kedar N Prasad |
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Institution: | (1) Department of Radiology, University of Colorado Medical Center, 80262 Denver, Colorado |
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Abstract: | Summary Adenosine 3′,5′-cyclic monophosphate (cyclic AMP) phosphodiesterase activity in mouse neuroblastoma cells in culture markedly
increased during exponential growth and reached a maximal level at confluency; whereas guanosine 3′, 5′-cyclic monophosphate
(cyclic GMP) phosphodiesterase activity only slightly but significantly increased under a similar experimental condition.
The increase in cyclic AMP phosphodiesterase activity was blocked by both cycloheximide and dactinomycin, whereas the increase
in cyclic GMP phosphodiesterase was blocked by only cycloheximide. When the confluent cells were replated at low density,
the cyclic nucleotide phosphodiesterase activity decreased; however, when they were plated at high cell density which equaled
confluency, the enzyme activity did not decrease. Unlike cyclic AMP phosphodiesterase activity, cyclic GMP phosphodiesterase
activity did not change significantly in prostaglandin E1-treated cells, but decreased in cells treated with the inhibitor of phosphodiesterase. Like cyclic AMP phosphodiesterase
activity, cyclic GMP phosphodiesterase activity also did not change in cells treated with serum-free medium, X-irradiation,
sodium butyrate and 6-thioguanine.
This work was supported by USPHS NS-09230, and DRG-1273 from Damon Runyon-Walter Winchell Cancer Fund. |
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Keywords: | neuroblastoma cells cyclic AMP phosphodiesterase cyclic GMP phosphodiesterase exponential confluent |
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