Somatic embryogenesis,plant regeneration,and the evaluation of camptothecin content in <Emphasis Type="Italic">Nothapodytes foetida</Emphasis> |
| |
Authors: | Email author" target="_blank">Devanand?P?FulzeleEmail author Ramesh?K?Satdive |
| |
Institution: | (1) Plant Biotechnology and Secondary Products Section, Nuclaar Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400 085 Trombay, Mumbai, India |
| |
Abstract: | Summary Somatic embryogenesis and plant regeneration have been achieved in Nothapodytes foetida, which is known for its rich source of anti-cancer and anti-AIDS alkaloids. Callus cultures were initiated from immature
zygotic embryos cultured on Murashige and Skoog's (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid, 6-benzyladenine
(BA), and kinetin. MS medium devoid of plant growth regulators favored the development of globular somatic embryos that differentiated
further into plantlets. Plantlet regeneration efficiency was effectively increased on MS medium supplemented with BA. Over
90% of the in vitro plantlets survived when transferred to the soil. Alkaloids were detected in different stages of somatic embryos, regenerated
plantlets, and different parts of the 2-yr-old regenerated plants. The somatic embryos contains camptothecin (0.011% dry weight.
DW) and 9-methoxycamptothecin (0.0028% DW). Two-yearold field-grown plants obtained from somatic embryos were analyzed and
contained higher levels of camptothecin (0.20% DW) and 9-methoxycamptothecin. (0.097% DW) accumulated in roots, followed by
stem and leaves. Alkaloids were quantified and identified by TLC and HPLC. |
| |
Keywords: | Nothapodytes foetida somatic embryos quinoline alkaloids camptothecin 9-methoxycamptothecin |
本文献已被 SpringerLink 等数据库收录! |
|