Micropropagation of the endangered <Emphasis Type="Italic">Kniphofia leucocephala</Emphasis> Baijnath |
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Authors: | Shelagh?A?Mccartan Email author" target="_blank">Johannes?van?StadenEmail author |
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Institution: | (1) Research Centre for Plant Growth and Development, School of Botany and Zoology, University of Natal Pietermaritzburg, Private Bag X01, 3209 Scottsville, South Africa |
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Abstract: | Summary The species, Kniphofia leucocephala is extant at only one location, Langepan, KwaZulu-Natal in South Africa, where the population is threatened by afforestation
and possibly grazing. Consequently, a continuous culture system was established as part of a program for the propagation and
re-introduction of plants into the wild. The efficiency of the system in terms of shoot multiplication and, particularly,
the frequency and rate of root initiation was strongly influenced by the concentration of benzyladenine in the shoot multiplication
medium. The optimum shoot multiplication medium for subsequent root initiation contained 2 mgl−1 (8.9 μM) benzyladenine alone. The shoots were successfully rooted and acclimatized. Approximately 200 shoots can be produced from
one shoot after five 4-wk cycles. Thus, large numbers of plantlets can be propagated in this continuous culture system, serving
conservation interests. |
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Keywords: | Asphodelaceae in vitro flowering in vitro propagation tissue culture |
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