In vitro differentiation and mineralization of human dental pulp cells induced by dentin extract |
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Authors: | Jun?Liu Taocong?Jin Helena?H?Ritchie Anthony?J?Smith Email author" target="_blank">Brian?H?ClarksonEmail author |
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Institution: | (1) Department of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 North University, 48109-1078 Ann Arbor, Michigan;(2) Department of Oral Biology, School of Dentistry, University of Birmingham, St. Chad's Queensway, B4 6NN Birmingham, UK |
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Abstract: | Summary In this study, the progenitor cells isolated from the human dental pulp were used to study the effects of ethylenediaminetetraacetic
acid-soluble dentin extract (DE) on their differentiation and mineralization to better understand tissue injury and repair
in the tooth. Mineralization of the matrix was increasingly evident at 14, 21, and 28 d after treatment with a mineralization
supplement (MS) (ascorbic acid AA], β-glycerophosphate β-GP]) and MS+DE. Real-time polymerase chain reaction results showed
type I collagen upregulation after the addition of MS+DE at 7 d. Alkaline phosphatase was downregulated after the mineralization
became obvious at 14 d. Bone sialoprotein was shown to be upregulated in the mineralized cell groups at all time points and
dentin sialophosphoprotein after 7 d. Core binding factor a 1 was upregulated by the treatment of MS and DE at 7, 14, and
21 d. These results indicated that the MS of AA, β-GP, and DE synergistically induced cell differentiation of pulp progenitor
cells into odontoblast-like cells and induced in vitro mineralization. |
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Keywords: | progenitor cell dentin sialophosphoprotein bone sialoprotein core binding factor a 1 mineralization |
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