Constructing an in vitro cornea from cultures of the three specific corneal cell types |
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Authors: | Adelheid I Schneider Katharina Maier-Reif Thomas Graeve |
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Institution: | 1. Roche Diagnostics GmbH, Im Nonnenwald 2, 82377, Penzberg 2. Drechselstr. 49, 89233, Neu-Ulm 3. Fraunhofer-Institute for Surface Technology and Biochemical Engineering, Nobelstra?e 12, 70569, Stuttgart
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Abstract: | Summary This paper presents a reliable method for establishing pure cultures of the three types of corneal cells. This is believed
to be the first time, corneal cells have been cultured from fetal pig corneas. Cell growth studies were performed in different
media. Subcultures of the three corneal cell types were passaged until the 30th generation without their showing signs of
senescence. For engineering an in vitro cornea, corneal epithelial cells were cultured over corneal stromal cells in an artificial
biomatrix of collagen with an underlying layer of corneal endothelial cells. The morphology, histology, and differentiation
of the in vitro cornea were investigated to determine the degree of comparability to the cornea in vivo. The in vitro construct
displayed signs of transition to an organotypic phenotype of which the most prominent was the formation of two basement membranes. |
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Keywords: | corneal cells cell growth studies cell characterization organ construct tissue engineering |
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