Isolation and culture of rat superior mesenteric artery smooth muscle cells |
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Authors: | Paul G McGuire Helen M Walker-Caprioglio Sally A Little Linda J McGuffee |
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Institution: | (1) Department of Anatomy, University of New Mexico School of Medicine, 87131 Albuquerque, New Mexico;(2) Department of Pharmacology, University of New Mexico School of Medicine, 87131 Albuquerque, New Mexico |
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Abstract: | Summary The structure and function of vascular smooth muscle cells have been extensively investigated with the aid of in vitro culture
techniques. The majority of studies have utilized aortic tissue as the source of cells. We present here a method for isolating
and culturing smooth muscle cells of the rat superior mesenteric artery, an elasto-muscular vessel that is structurally and
functionally different from the aorta. Cells were isolated from partially digested explants and characterized by immunochemical
and biochemical techniques. Unlike cultured fibroblasts, the cultured cells stained positive for smooth muscle specific actin.
The cells also produced laminin and type IV collagen in culture. This method provides a means for the isolation of large numbers
of viable smooth muscle cells from the superior mesenteric artery which can be propagated in culture for in vitro study. |
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Keywords: | smooth muscle culture matrix |
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