Dynamics of differentiation in human epidermoid squamous carcinoma cells (A431) with continuous,long-term γ-IFN treatment |
| |
Authors: | Jeanette Ridge Jacqueline Muller Philip Noguchi Esther H Chang |
| |
Institution: | (1) Center for Biologics Evaluation and Research, FDA, Bldg. 29A, Rm. 1C-17, 8800 Rockville Pike, 20892 Bethesda, Maryland;(2) Department of Pathology, USUHS, 20814 Bethesda, Maryland |
| |
Abstract: | Summary We investigated the long-term effects of continuous gamma interferon (γ-IFN) treatment on A431, a human squamous carcinoma
cell line. Cells were grown in an in vitro culture system, which over time produces cohesive cell masses (“tumoroids”) exhibiting
three-dimensional, histotypically differentiated structures, e.g., keratin “pearls,” intercellular bridges (desmosomes), elongated
flattened cells (squames) and stratification. The effects of γ-IFN on cell growth, morphology and stage of differentiation
were assessed at different treatment times by light and electron microscopy and by immunohistochemical staining using antibodies
to keratins 1 and 14 and to filaggrin, markers of specific stages of keratinocyte differentiation. Our results show that A431
cells have the capacity for spontaneous differentiation, that this capacity is significantly enhanced and accelerated by γ-IFN
treatment leading to terminal differentiation and extensive cell death by 2 wk. Despite continuous exposure to IFN, a small
number of viable, undifferentiated cells remain. Their proliferation, evident by 3 wk, reconstitutes the tumoroid which once
again contains the full range of differentiating cell types. |
| |
Keywords: | human squamous carcinoma cells (A431) γ -IFN differentiation long-term in vitro culture |
本文献已被 SpringerLink 等数据库收录! |
|