Excitability and isometric contractile properties of mammalian skeletal muscle constructs engineered in vitro |
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Authors: | Robert G Dennis Paul E Kosnik II |
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Institution: | (1) Muscle Mechanics Laboratory, Institute of Gerontology, University of Michigan, 300N. Ingalls, Room 901, 48109-2007 Ann Arbor, Michigan;(2) Department of Biomedical Engineering, University of Michigan, 300 N. Ingalls, Room 901, 48109-2007 Ann Arbor, Michigan |
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Abstract: | Summary Our purpose was to engineer three-dimensional skeletal muscle tissue constructs from primary cultures of adult rat myogenic
precursor cells, and to measure their excitability and isometric contractile properties. The constructs, termed myooids, were
muscle-like in appearance, excitability, and contractile function. The myooids were 12 mm long and ranged in diameter from
0.1 to 1 mm. The myooids were engineered with synthetic tendons at each end to permit the measurement of isometric contractile
properties. Within each myooid the myotubes and fibroblasts were supported by an extracellular matrix generated by the cells
themselves, and did not require a preexisting scaffold to define the size, shape, and general mechanical properties of the
resulting structure. Once formed, the myooids contracted spontaneously at approximately 1 Hz, with peak-to-peak force amplitudes
ranging from 3 to 30 μN. When stimulated electrically the myooids contracted to produce force. The myooids (n=14) had the following mean values:
diameter of 0.49 mm, rheobase of 1.0 V/mm, chronaxie of 0.45 ms, twitch force of 215 μN, maximum isometric force of 440 μN, resting baseline force of 181 μN, and specific force of 2.9kN/m2. The mean specific force was approximately 1% of the specific force generated by control adult rat muscle. Based on the functional
data, the myotubes in the myooids appear to remain arrested in an early developmental state due to the absence of signals
to promote expression of adult myosin isoforms. |
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Keywords: | tissue culture tissue engineering organoid myooid myogenesis organogenesis myocytes force measurement muscle contraction cell culture myotubes |
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