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Protoplast isolation in the marine brown alga Dictyopteris prolifera (Dictyotales) |
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| Authors: | T Fujimura T Kawai T Kajiwara Y Ishida |
| Institution: | (1) Laboratory of Marine Biotechnology, Shiono Koryo Kaisha, Ltd., Niitaka, Yodogawa-ku, 532 Osaka, Japan;(2) Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yoshida, 753 Yamaguchi, Japan;(3) Department of Fisheries, Faculty of Agriculture, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku, 606 Kyoto, Japan |
| Abstract: | Protoplasts were isolated from thalli of Dictyopteris prolifera using a mixture of crude enzymes from vicera of live oysters (Crassostrea gigas) and the following commercial enzymes: an abalone enzyme, cellulase, polygalacturonase and hemicellulase. The enzyme mixtures produced up to 3.3 × 107 cells per l g of tissue fresh weight. The conversion to protoplasts of the cells was about 100% using the oyster enzyme or the abalone enzyme alone. The optimum pH for protoplast isolation was 6.0 and 20 hours were required for conversion to protoplasts. |
| Keywords: | Crassostrea gigas Dictyopteris prolifera Marine brown alga Protoplast Single cell |
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