Expression of soybean b-1,3-endoglucanase cDNA and effect on disease tolerance in kiwifruit plants |
| |
| Authors: | Y Nakamura H Sawada S Kobayashi I Nakajima M Yoshikawa |
| |
| Institution: | (1) Persimmon and Grape Research Center, National Institute of Fruit Tree Science, Akitsu, Hiroshima 729-2494, Japan, JP;(2) National Institute of Agriculture Environment Science, Tsukuba Kannondai 3-1-1, Ibaraki 305-8604, Japan, JP;(3) Department of Botany, Faculty of Science, Hokkaido University, Sapporo, Hokkaido 060, Japan, JP |
| |
| Abstract: | Kiwifruit was transformed with a soybean β-1,3-endoglucanase (EC 3.2.1.39) cDNA under the control of the cauliflower mosaic virus (CaMV) 35S RNA promoter. The introduced
gene was expressed in young leaves of the transformants. Assays of protein extracts from young leaves showed an increase in
enzyme activity in many transformants, the transformant with the highest level of enzyme activity having an about sixfold
increase over the control plants. When leaves from control and three transformants were inoculated with Botrytis cinerea, which causes gray mold disease, the disease lesion areas for two transformants were smaller than on control plants.
Received: 5 March 1998 / Revision received: 19 October 1998 / Accepted: 27 October 1998 |
| |
| Keywords: | Agrobacterium Kiwifruit β -1 3-Endo-glucanase Transformation Disease tolerance |
| 本文献已被 SpringerLink 等数据库收录！ |
|
