Plant regeneration from axillary buds of a mature tree of Ficus religiosa |
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Authors: | S R Deshpande P C Josekutty G Prathapasenan |
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Institution: | (1) Department of Botany, Faculty of Science, M. S. University of Baroda, Vadodara 390 002 Gujarat, India, IN |
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Abstract: | Dormant axillary buds of nodal explants collected from a mature (35-year-old) tree of Ficus religiosa L. sprouted on MS medium supplemented with 6-benzyladenine (BA, 5 mg l–1) and indole-3-butyric acid (IBA, 0.2 mg l–1 ) within 4 days. Multiple shoots were obtained when these explants were transferred to MS medium containing 1.5 mg l–1 BA and 1.5 mg l–1 adenine sulphate (ADS). These multiple shoots (1 cm) were allowed to elongate to a height of 4–5 cm by maintaining them on
MS medium containing 2 mg l–1 BA, 0.5 mg l–1 1-naphthalene acetic acid (NAA), and activated charcoal (0.3% w/v). Nodal segments taken from these in vitro-proliferated
shoots yielded multiple shoots when cultured on the multiple shoot-inducing MS medium mentioned above. Root induction in these
shoots (4–5 cm in height) was achieved by transferring them onto MS medium supplemented with 2 mg l–1 IBA and 0.1 mg l–1 NAA for 1 week; upon transfer to half-strength MS basal medium these shoots exhibited root proliferation. These rooted plantlets
were successfully established in soil after a short period of acclimatization.
Received: 17 April 1997 / Revision received: 2 September 1997 / Accepted: 20 September 1997 |
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Keywords: | Micropropagation Ficus religiosa L Mature tree Nodal explants |
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