Cryopreservation of shoot tips from in vitro plants of sweet potato [Ipomoea batatas (L.) Lam.] by vitrification |
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| Authors: | J C Pennycooke L E Towill |
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| Institution: | (1) Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523, USA, US;(2) USDA/ARS National Seed Storage Laboratory, 1111 S. Mason St., Fort Collins, CO 80521, USA e-mail: ltowill@lamar.colostate.edu Fax: 970–221–1427, US |
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| Abstract: | Routine cryopreservation of shoot tips from sweet potato Ipomoea batatas (L.) Lam] has been hampered by their survival variability after cryogenic exposure. We examined the effects of light conditions
on stock plants, sucrose preculture and cryoprotectant loading on survival after vitrification using PVS2 solution. The survival
of vitrified sweet potato shoot tips cooled to approximately –208 °C was increased by preculturing with 0.3 M sucrose for 24 h at 22 °C. Survival was also enhanced by excising shoot tips immediately after the 8-h dark photoperiod.
The best survival after cryogenic exposure was obtained using 2 M glycerol +0.4 M sucrose for 1 h at 22 °C followed by dehydration with PVS2 for 16 min at 22 °C. Rapid cooling was used and achieved by
the immersion of foil strips into partially solidified nitrogen. Successfully vitrified and warmed shoot tips directly developed
shoots on a medium containing 1 μM NAA, 0.5 μM BA and 0.1 μM kinetin with only minimum callus formation. Shoot formation occurred in all surviving shoot tips. This procedure shows promise
for cryopreserving sweet potato shoot tips.
Received: 2 March 1999 / Revision received: 21 September 1999 / Accepted: 29 September 1999 |
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| Keywords: | Shoot tips Cryopreservation Vitrification Sweet potato [Ipomoea batatas (L) Lam ] |
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