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Transfer of a grapevine stilbene synthase gene to rice (Oryza sativa L.) |
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| Authors: | P Stark-Lorenzen B Nelke G Hänßler H P Mühlbach J E Thomzik |
| Institution: | Max-Planck-Institut für Züchtungsforschung, D-50829 K?ln, Germany, DE Agrochemical Division, Research/Biotechnology, Bayer AG, D-51368 Leverkusen-Bayerwerk, Germany, DE Institut für Allgemeine Botanik, Universit?t Hamburg, Ohnhorststra?e 18, D-22609 Hamburg, Germany, DE |
| Abstract: | A gene derived from grapevine (Vitis vinifera) coding for stilbene synthase has been transferred into protoplasts of the commercially important japonica rice cultivar Nipponbare using PEG-mediated direct gene transfer. Transgenic plants were regenerated from calli selected on kanamycin. Southern blot analysis of genomic DNA isolated from regenerants and progeny plants demonstrated that the stilbene synthase gene is stably integrated in the genome of transgenic rice plants and inherited in the offspring. The transient formation of stilbene-synthase-specific mRNA shortly after inoculation with the fungus of the rice blast Pyricularia oryzae has demonstrated that the grapevine stilbene synthase promoter is also active in monocotyledonous plants. Preliminary results indicate an enhanced resistance of transgenic rice to P. oryzae. Received: 1 July 1996 / Revision received: 5 November 1996 / Accepted: 30 November 1996 |
| Keywords: | Oryza sativa Pyricularia oryzae Transformation Stilbene synthase Phytoalexin |
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