Sterol glucosylation by the plasma membrane from cotyledons of Phaseolus aureus |
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| Authors: | Marie-Andrée Hartmann-Bouillon Bernard Walter Eliane Lazar |
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| Institution: | (1) Laboratoire de Biochimie Végétale, Institut de Botanique de l'Université Louis Pasteur, 28, rue Goethe;(2) Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire du CNRS, 15, rue Descartes, F-6700 Strasbourg, France |
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| Abstract: | Membrane fractions were isolated from dark grown cotyledons of Phaseolus auneus by differential and sucrose density gradient centrifugation. Endoplasmic reticulum-, Golgi apparatus- and plasma membrane-rich fractions were identified by their respective enzymic activities and tested for their ability to transfer glucose from UDP-glucose to endogenous sterols to form steryl glucosides. The glucosyltransferase activity was shown to be located mainly at the plasma membrane.ABBREVIATIONS SG
steryl glucoside
- ASG
acylated steryl glucoside
- UDP-glc
Uridine diphosphoglucose |
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