Differential expression of Phaseolus vulgaris genes induced during the interaction with Rhizoctonia solani |
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Authors: | Guerrero-González M L Rodríguez-Kessler M Rodríguez-Guerra R González-Chavira M Simpson J Sanchez F Jiménez-Bremont J F |
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Institution: | (1) Divisi?n de Biolog?a Molecular, Instituto Potosino de Investigaci?n Cient?fica y Tecnol?gica, Camino a la Presa de San Jos? 2055 AP 3-74 Tangamanga, CP 78216, San Lu?s Potos?, M?xico;(2) Departamento de Biolog?a Molecular de Plantas, Instituto de Biotecnolog?a, Universidad Nacional Aut?noma de M?xico, Av. Universidad 2001, Colonia Chamilpa, CP 62210, Cuernavaca, Morelos, M?xico;(3) Campo Experimental General Ter?n, Instituto Nacional de Investigaciones Forestales, Agr?colas y Pecuarias, AP No. 3. Km. 31 Carretera Montemorelos-China, CP 67400, General Ter?n, Nuevo Le?n, M?xico;(4) Campo Experimental Baj?o, Instituto Nacional de Investigaciones Forestales, Agr?colas y Pecuarias, Km. 6.5 Carretera Celaya-San Miguel de Allende, AP 112, Celaya, Guanajuato, M?xico;(5) Departamento de Ingenier?a Gen?tica, Centro de Investigaci?n y de Estudios Avanzados del Instituto Polit?cnico Nacional, Unidad Irapuato, AP 629, Irapuato, Guanajuato, M?xico; |
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Abstract: | Common bean (Phaseolus vulgaris L.) is the most important grain legume for direct human consumption; however, bean production is affected by several diseases
such as Rhizoctonia root rot. Few bean cultivars have been identified that effectively resist the attack of this fungus. Herein,
we used the P. vulgaris Pv-2094 landrace, which is less susceptible to Rhizoctonia root rot, for the construction of a suppressive subtractive hybridization
cDNA library in order to isolate plant defense-related genes. Total RNAs obtained after 8 and 16 h from inoculated and non-inoculated
roots with R. solani Kühn, were used as the source of the “tester” and the “driver” samples, respectively. A total of 136 unigenes were obtained
and classified into 12 functional categories. Six unigenes were selected to analyze for differential expression by qRT-PCR,
including a receptor-like kinase (PvRK20-1), an acid phosphatase associated to defense (PA), a pathogenesis related protein (PR1), an ethylene responsive factor (ERF), a polygalacturonase inhibitor protein (PGIP), and an alpha-dioxygenase (α-DOX). These genes were found to be differentially expressed in a time-dependent manner in bean roots during the interaction with
R. solani. Data generated from this study will contribute to the understanding of the molecular mechanisms associated with plant defense
against root rot in common bean. |
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