In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation |
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Authors: | Sanjay Saxena |
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Institution: | (1) Tata Energy Research Institute, 90 Jor Bagh, 110003 New Delhi, India |
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Abstract: | An efficient protocol has been developed for the in vitro propagation of Bambusa tulda through shoot proliferation. Shoots from 3-week-old aseptically grown seedlings were used to initiate cultures. Multiple shoots were obtained on liquid Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (8×10–6M) and kinetin (4×10–6M). Continuous shoot proliferation at a rate of 4–5 fold every three weeks was achieved through forced axillary branching. More than 90% of the shoots could be rooted on a modified MS medium containing indoleacetic acid (1×10–5M) and coumarin (6.8×10–5M). Following simple hardening procedures, the in vitro raised plants were transferred to the soil with more than 80% success.Abbreviations BAP
6-benzylaminopurine
- 2-ip
6-,-dimethylallylaminopurine
- Kn
kinetin
- IAA
3-indoleacetic acid
- IBA
3-indolebutyric acid
- NAA
1-naphthaleneacetic acid |
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Keywords: | |
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