Isolation and functional characterization of a novel rice constitutive promoter |
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Authors: | Juan Li Rong-Fang Xu Rui-Ying Qin Hui Ma Hao Li Ying-Ping Zhang Li Li Peng-Cheng Wei Jian-Bo Yang |
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Institution: | 1. Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences, Hefei, 230031, China 2. Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, China 3. Institute of Agricultural Engineering, Anhui Academy of Agricultural Sciences, Hefei, 230031, China
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Abstract: | Key message A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Abstract Monocot constitutive promoter is an important resource for crop transgenic engineering. In this report, we isolated a novel promoter, Oscon1 promoter (P OsCon1 ), from the 5′ upstream region of a constitutively expressed rice gene OsDHAR1. In P OsCon1 ::GUS transgenic rice, we showed that P OsCon1 had a broad expression spectrum in all tested tissues. The expression of the promoter was further analyzed in comparison with the previously characterized strong constitutive promoters. P OsCon1 exhibited comparable activity to OsCc1, OsAct1 or ZmUbi promoters in most tissues, and more active than 35S promoter in roots, seeds, and calli. Further quantitative assays indicated that P OsCon1 activity was not affected by developmental stages or by environmental factors. Further, 5′-deletions analysis indicated that the distinct regions might contribute to the strong expression of P OsCon1 in different tissues. Overall, our results suggest that P OsCon1 is a novel constitutive promoter, which could potentially use in transgenic crop development. |
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