Morphological changes in homeotic cytoplasmic male-sterile carrots combined with fertile cytoplasm by asymmetrical cell fusion |
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Authors: | T Yamamoto Y Nakajima K Oeda |
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Institution: | (1) Biotechnology Laboratory, Sumitomo Chemical Co., Ltd, 4-2-1 Takatsukasa, Takarazuka Hyogo 665-8555, Japan Fax: (+81) 797-74-2133 e-mail: oeda@sc.sumitomo-chem.co.jp, JP |
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Abstract: | The cytoplasmic male-sterile (CMS) carrot (Daucus carota ssp. sativus) with the petaloid phenotype was asymmetrically fused with eight different fertile cytoplasms to convert the CMS to a fertile
state. Restoration to the fertile phenotype was successful with an over 20% efficiency. Cybrids with brown anther sterile,
incomplete petaloid sterile, or "combined flower" fused on the same axis were also observed. Restricted DNA fragment patterns
revealed that the mitochondrial genome organizations of the cybrids were not identical to those of their parents but were
of an intermediate type. Repeated cell fusion to introduce two different foreign cytoplasms into the CMS cytoplasm was effective
for obtaining fertile plants. The role of mitochondrial factors which regulate flower organ morphogenesis was demonstrated.
Received: 14 December 1998 / Revision received: 7 March 1999 · Accepted: 3 June 1999 |
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Keywords: | Daucus carota L Mitochondrial DNA Repeated cell fusion |
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