Somatic embryogenesis from mature embryo-derived leaflets of peanut (Arachis Hypogaea L.) |
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Authors: | K Chengalrayan Sangeeta S Sathaye Sulekha Hazra |
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Institution: | (1) Division of Plant Tissue Culture, National Chemical Laboratory, 411 008 Pune, India |
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Abstract: | Summary Embryogenic masses were obtained from immature leaves of peanut (Arachis hypogaea L.) cultured on a medium containing 20 mg/l 2,4-D. Somatic embryos developed from these masses following transfer to a medium containing 3 mg/l 2,4-D. The embryo morphology was quite variable. Following transfer to hormone-free medium, these embryos germinated. Shoot elongation was obtained in 25% of the embryos following transfer to a medium supplemented with 0.5 mg/l each of BAP and Kn. The plants grown in vitro by this method survived in sand:soil mixture and were grown to maturity.Abbreviations ABA
abscisic acid
- BAP
6-benzyl amino purine
- 2,4-D
2,4 dichlorophenoxyacetic acid
- GA3
gibberellic acid
- Kn
kinetin
- NAA
1-naphthaleneacetic acid
- 2,4,5-T
2,4,5-trichlorophenoxyacetic acid
- Z
zeatin |
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Keywords: | Mature embryo derived Conversion Arachis hypogaea |
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