Somatic embryogenesis from leaf- and petiole-derived callus of Vitis rupestris |
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Authors: | Lucia Martinelli Paola Bragagna Valentino Poletti Attilio Scienza |
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Institution: | (1) Laboratorio Biotecnologie, Istituto Agrario, via Mach 1, I-38010 San Michele all'Adige, TN, Italy;(2) Istituto Coltivazioni Arboree, Università degli Studi di Milano, Italy |
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Abstract: | Somatic embryogenesis from leaf- and petiole-derived calli of Vitis rupestris was obtained with an efficiency of 3.2% and 4.2% of plated explants, respectively on two combinations of 6-benzyladenine and 2,4-dichlorophenoxyacetic acid (1/0.1 and 1/1 mgl–1) added to MS medium. Embryogenic callus, embryo subcultures and somatic embryogenesis from somatic embryos were obtained either in the presence of 1 mgl–1 indole-3-acetic acid or 0.1 mgl–1 indole-3-butyric acid added to MS or NN media. Within a 4-month culture, embryo germination occurred at a frequency of 13% of explanted embryos when chilling at 4°C was provided for two weeks and a combination of 6-benzyladenine (1 mgl–1) with indole-3-butyric acid (0.1 mgl–1) was added to NN medium supplemented with casein hydrolysate (250 mgl–1). A higher frequency (51%) was obtained in a longer culture time (9 months) when only indole-3-butyric acid was present in the medium and in absence of chilling.Abbreviations BA
6-benzyladenine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- MS
Murashige and Skoog (1962)
- NN
Nitsch and Nitsch (1969)
- NOA
2-naphthoxyacetic acid |
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Keywords: | Germination grape in vitro regeneration |
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