Development of an efficient Agrobacterium-mediated transformation system for Brassica carinata |
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Authors: | V Babic R S Datla G J Scoles W A Keller |
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Institution: | (1) Department of Crop Science and Plant Ecology, University of Saskatchewan, 51 Campus Dr., Saskatoon, Saskatchewan, S7N 5A8, Canada, CA;(2) Plant Biotechnology Institute, National Research Council, 110 Gymnasium Place, Saskatoon, Saskatchewan, S7N 0W9, Canada Fax no.: +1-306-975-4839 E-mail:wkeller@pbi.nrc.ca, CA |
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Abstract: | Shoot organogenesis and plant regeneration were readily achieved from cotyledonary petioles and hypocotyls of Brassica carinata. These explants were used for Agrobacterium-mediated transformation. A construct containing the selectable marker genes, neomycin phosphotransferase II, phosphinothricin
acetyl transferase and the reporter gene β-glucuronidase, under the control of a tandem 35S promoter, was used for transformation. Although transformation was achieved
with both cotyledonary petioles and hypocotyls, cotyledonary petioles responded best, with 30–50% of the explants producing
GUS-positive shoots after selection on 25 mg/l kanamycin. Direct selection on L-phosphinothricin also produced resistant shoots
but at a lower frequency (1–2%).
Received: 9 April 1997 / Revision received: 3 July 1997 / Accepted: 30 July 1997 |
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Keywords: | Brassica Mustard Transgenic Transformation Regeneration |
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