Somaclonal-variation-induced aluminum-sensitive mutant from an aluminum-inbred maize tolerant line |
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Authors: | D H Moon L M M Ottoboni A P Souza S T Sibov M Gaspar P Arruda |
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Institution: | Departamento de Genética e Evolu??o, Instituto de Biologia, Estadual de Campinas, Brazil, BR Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil, BR
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Abstract: | Somaclonal-variation-induced multiple mutations were observed in a progeny of the S1587 plant, regenerated from type I calli
of the aluminum-tolerant inbred maize line Cat-100-6. After five generations of self-pollination, 14 progeny families of the
S1587 somaclone were found to show aluminum toxicity symptoms with altered root tip morphology and reduced primary root growth.
The most sensitive progeny, S1587-17, was crossed to the Cat-100-6 inbred line. The parental lines and the F1 were tested
in nutrient solutions containing an aluminum activity gradient of 0–93 ⋅ 10–6. The heterozygote behaves like the tolerant parent at aluminum activities up to 40 ⋅ 10–6 and showed an intermediate phenotype at higher aluminum concentrations. Histological sections of aluminum-treated roots from
tolerant and sensitive plants stained with hematoxylin, an aluminum marker, showed a progressive destruction of the root tip
of the aluminum-sensitive genotype over time and indicated that tolerance in Cat-100-6 could be due to an aluminum exclusion
mechanism. Segregation analysis of the F2 and backcross to the sensitive parent based on root morphology of plants subjected
to an aluminum activity of 30 ⋅ 10–6 showed the typical 3:1 and 1:1 tolerant:sensitive segregation ratios, respectively, indicating that tolerance in the Cat-100-6
inbred maize line is controlled by a single nuclear, semidominant gene, named Alm1.
Received: 9 May 1996 / Revision received: 24 February 1997 / Accepted: 8 March 1997 |
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Keywords: | Maize Aluminum tolerance Somaclonal variation |
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