Incorporation of haem into phycocyanobilin in levulinic acid treated Cyanidium caldarium |
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| Authors: | A Schuster H -P Köst W Rüdiger J A Holroyd S B Brown |
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| Institution: | (1) Botanisches Institut der Universität München, Menzinger Straße 67, D-8000 München 19, FRG;(2) Department of Biochemistry, The University of Leeds, LS2 9JT Leeds, UK |
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| Abstract: | Cells of the red alga Cyanidium caldarium were preincubated with 5 mmol 1–1 levulinic acid (LA) and subsequently incubated with 14C-labelled haem (5.67 Bq nmol–1). Phycocyanin was isolated. The specific radio-activity of its chromophore phycocyanobilin (PCB) was determined after cleavage and purification by thin layer chromatography. The percentage of PCB formed from labelled haem within 0.5 h was considerably higher in LA treated cells than in non-treated controls. This difference disappeared after prolonged incubation (16.5 h) with haem. The results are interpreted as possible incorporation of haem into preexisting apoprotein.Abbreviations LA
levulinic acid
- PCB
phycocyanobilin |
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