Plant regeneration in Arachis pintoi (Leguminosae) through leaf culture |
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Authors: | H Y Rey A M Scocchi A M Gonzalez L A Mroginski |
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Institution: | (1) Instituto de Botánica del Nordeste (IBONE). Facultad de Ciencias Agrarias (UNNE), Casilla Correos 209, 3400 Corrientes, Argentina Fax: +54-3783-427131 e-mail: ibone@espacio.com.ar, AR |
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Abstract: | Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated
from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination
for plant regeneration through organogenesis was an initial medium composed of 10 mg/l NAA+1 mg/l BA followed by transfer
of the callus to a shoot induction medium (MS+1 mg/l BA). Rooting of regenerated shoots was readily achieved by culture on
MS+0.01 mg/l NAA. Embryogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with PICL in combination
with KIN, ZEA, BA or 2iP, and the most suitable combinations were 20 mg/l PICL+1 mg/l BA or 2iP. When pieces of embryogenic
callus were subcultured on MS+1 mg/l BA, somatic embryos were differentiated and developed further into well-developed plants
in MS+1 g/l AC followed by MS medium devoid of plant growth regulators.
Received: 29 April 1999 / Revision received: 24 November 1999 / Accepted: 18 December 1999 |
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Keywords: | Somatic embryogenesis Organogenesis Arachis pintoi Plant regeneration |
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