Expression and inheritance of foreign genes in transgenic peanut plants generated byAgrobacterium-mediated transformation |
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| Authors: | M Cheng Z Li J W Demski R L Jarret |
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| Institution: | (1) Department of Plant Pathology, University of Georgia, Georgia Station, 1109 Experiment Street, 30223 Griffin, GA, USA;(2) USDA-ARS Plant Genetic Resources Conservation Unit, Georgia Station, 1109 Experiment Street, 30223 Griffin, GA, USA;(3) Present address: Ceregen, 700 Chesterfield Parkway North/GG4H, 63198 St. Louis, MO, USA |
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| Abstract: | To evaluate and characterize the stability of traits transferred viaAgrobacterium transformation, foreign gene expression must be examined in sexually derived progeny. The objective of this study was to analyze three transgenic peanut plants, 1-10, 12-1, and 17-1, for the inheritance and expression of their foreign genes. Segregation ratios for the introduced genes in T2 plants gave either 100% or 3:1 expression of the  -glucuronidase (GUS) gene, demonstrating recovery of both homozygous and heterozygous T1 plants. Fluorometric GUS assay in T1 and T2 generations of all three plants showed that the GUS gene was stably expressed in the progeny. DNA analyses showed 100% concordance between the presence of the foreign gene and enzyme activity. Our results demonstrate that transgenes in peanut introduced byAgrobacterium can be inherited in a Mendelian manner.Abbreviations
GUS
-Glucuronidase
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MS
Murashige and Skoog
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MU
4-Methylumbelliferone
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NPTII
Neomycin phosphotransferase II |
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| Keywords: | Transgenic peanut Agrobacterium Transformation Transgene expression Transgene inheritance |
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