Positive selection: a plant selection principle based on xylose isomerase, an enzyme used in the food industry |
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Authors: | A Haldrup S G Petersen F T Okkels |
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Institution: | (1) Danisco Biotechnology, 1 Langebrogade, P. O. Box 17, DK-1001 Copenhagen K, Denmark, DK |
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Abstract: | A new method for the selection of transgenic plants has been developed. It is based upon selection of transgenic plant cells
expressing the xylA gene from Streptomyces rubiginosus, which encodes xylose isomerase, on medium containing xylose. The xylose isomerase selection system was tested in potato
and the transformation frequency was found to be approximately ten fold higher than with kanamycin selection. The level of
enzyme activity in the transgenic plants selected on xylose was 5- to 25-fold higher than the enzyme activity in control plants.
Potato transformants were stable over two generations in Southern blotting analysis. This novel selection system is more efficient
than the traditionally used kanamycin-based selection systems. In addition, the xylose isomerase system is independent of
antibiotic or herbicide resistance genes, but depends on an enzyme that is generally recognized as safe for use in the starch
industry and which is already being widely utilized in specific food processes.
Received: 13 August 1997 / Revision received: 26 November 1997 / Accepted: 15 December 1997 |
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Keywords: | Plants Selectable marker Xylose isomerase Streptomyces rubiginosus |
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