Easy determination of ploidy level in Arabidopsis thaliana plants by means of pollen size measurement |
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| Authors: | Thomas Altmann Brigitte Damm Wolf B Frommer Thomas Martin Peter C Morris Dieter Schweizer Lothar Willmitzer Renate Schmidt |
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| Institution: | (1) Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, 14195 Berlin, Germany;(2) Institut for Botanik, Universität Wien, Rennweg 14, A-1030 Wien, Austria;(3) Present address: MOGEN International nv, Einsteinweg 97, 2333 CB Leiden, The Netherlands;(4) Present address: IPSR Cambridge Laboratory, John Innes Centre, Colney Lane, NR4 7UJ Norwich, UK |
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| Abstract: | Summary Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.Abbreviations AM
Arabidopsis medium
- ANOVA
analysis of variance
- DAPI
4,6-Diamidino-2-phenylindole
- PEG
polyethyleneglycol |
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| Keywords: | Transgenic Arabidopsis Ploidy Pollen size |
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