Identification of hazelnut (Corylus avellana) cultivars by RAPD analysis |
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| Authors: | U Galderisi M Cipollaro G Di Bernardo L De Masi G Galano A Cascino |
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| Institution: | (1) C. R. I. S. C. E. B. and Istituto di Farmacologia e Tossicologia, 2.a Università degli Studi di Napoli, Facoltà di Medicina e Chirurgia, Via Costantinopoli 16, I-80138 Naples, Italy e-mail: cascino@unina.it Fax:.+39-81-5665879, IT |
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| Abstract: | The random amplified polymorphic DNA (RAPD) technique offers a useful tool to detect DNA polymorphisms. It can also be used
to distinguish different clones and cultivars. We have developed a comprehensive RAPD-based procedure for the routine molecular
typing of various plants. Here we report the application of this technique for the correct identification of six hazelnut
cultivars (Corylus avellana) widespread in the Campania region (south Italy). The analysed hazelnut cultivars were successfully distinguished by their
RAPD fingerprints using the DNA primers U2, U3, U4, U11 and U14. However, in each cultivar we observed very low genetic heterogeneity
among the clonal variants. Since this technique is among the simplest and easiest methods used to fingerprint DNA, it could
be easily transferred to less sophisticated laboratory infrastructures (e.g. outstations of crop regulatory agencies).
Received: 20 December 1997 / Revision received: 6 August 1998 / Accepted: 13 November 1998 |
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| Keywords: | PCR RAPD analysis Corylus avellana Cultivars |
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