Dehydrin genes and their expression in recalcitrant oak (<Emphasis Type="Italic">Quercus robur</Emphasis>) embryos |
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Authors: | Vanda Šunderlíková Ján Salaj Dieter Kopecky Terézia Salaj Eva Wilhem Ildikó Matušíková |
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Institution: | (1) Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 950 07 Nitra 1, Slovak Republic;(2) Department of Health and Environment/Bioresources, Austrian Research Centers GmbH-ARC, 2444 Seibersdorf, Austria |
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Abstract: | In this work, three dehydrin genes, QrDhn1, QrDhn2, QrDhn3, were isolated from recalcitrant oak (Quercus robur). Their expression pattern was analyzed in both zygotic and somatic embryos as well as in vegetative tissues exposed to different
kinds of abiotic stresses including desiccation, osmotic stress, and chilling. The QrDhn1 gene encoding for YnSKn type dehydrin was expressed during later stages of zygotic embryo development but in somatic embryos only when exposed to
osmotic or desiccation stress. In contrast, the other two oak dehydrin genes encoding for putative Kn type dehydrins were expressed only in somatic embryos (both not-treated and osmotically stressed) and leaves of oak seedlings
exposed to desiccation. Behavior of these genes suggests that different dehydrins are involved in processes of seed maturation
and response to altered osmotic (water status) conditions in somatic embryos. Revealing further members of dehydrin gene family
in recalcitrant oak might contribute to clarify non-orthodox seed behavior as well as identify mechanisms contributing to
desiccation tolerance in plants. |
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Keywords: | LEA-2 genes Seed desiccation Somatic embryos Zygotic embryos |
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