Assessment of genetic stability of the germplasm lines of medicinal plant <Emphasis Type="Italic">Scutellaria baicalensis</Emphasis> Georgi (Huang-qin) in long-term,in vitro maintained cultures |
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Authors: | Ali R Alan Hongyan Zeng Akym Assani Wendy L Shi Hannah E McRae Susan J Murch Praveen K Saxena |
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Institution: | (1) Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada, N1G 2W1;(2) Department of Chemistry, I.K. Barber School of Arts and Sciences, University of British Columbia Okanagan, Kelowna, BC, Canada, V1V 1V7 |
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Abstract: | An approach of combining flow cytometry (FCM) analysis with morphological and chemical profiling was used to assess the genetic
stability and bioactive compound diversity in a Scutellaria baicalensis Georgi (Huang-qin) germplasm collection that was clonally maintained in in vitro for a period of over 6 years. Based on the
FCM analysis of nuclei samples from young shoots, the nuclear DNA content of S. baicalensis was calculated as 0.84 pg/2C. FCM analysis showed no significant variation in the nuclear DNA contents and ploidy levels
in the long-term in vitro maintained germplasm lines. Germplasm lines, acclimatized to ex vitro conditions, exhibited distinctive
plant growth and bioactive compound production capacities. The high level of genetic stability observed in in vitro maintained
S. baicalensis lines opens up a variety of opportunities such as allowing long-term aseptic preservation and easy distribution of well-characterized
germplasm lines of this medicinal plant species. This study represents a novel approach for continuous maintenance, monitoring,
and production of medicinal plant tissues with specific chemistry. |
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Keywords: | Antioxidant Flavonoid Flow cytometry Nuclear DNA content Ploidy Scutellaria baicalensis |
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