Correlation of cotyledonary node shoot proliferation and somatic embryoid development in suspension cultures of soybean (Glycine max L. Merr.) |
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| Authors: | H R Kerns U B Barwale M M Meyer Jr J M Widholm |
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| Institution: | (1) Department of Horticulture, University of Illinois at Urbana-Champaign, 61801, IL, USA;(2) Department of Agronomy, University of Illinois at Urbana-Champaign, 61801, IL, USA |
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| Abstract: | Suspension cultures of soybean were initiated from hypocotyl or cotyledon callus tissue of several soybean genotypes. When these were grown on L2 medium with 0.4 mg/liter 2,4-D several genotypes produced numerous embryoids while others produced only a few such structures. Due to internal anatomy, no embryoid developed into a complete plant. A genotype's propensity to form normal appearing embryoids was correlated with the ability to proliferate shoots at the cotyledonary node on a medium with benzylaminopurine as determined in previous testing.Abbreviations 2,4-D
2,4-Dichlorophenoxyacetic acid
- BAP
Benzylaminopurine
- L2
Phillips and Collins (1979) legume medium |
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