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Fungal and bacterial disease resistance in transgenic plants expressing human lysozyme
Authors:H Nakajima  T Muranaka  F Ishige  K Akutsu  K Oeda
Institution:(1) Biotechnology Laboratory, Sumitomo Chemical Co. Ltd., 2-1, 4-chome, Takatsukasa, Takarazuka, Hyogo 665, Japan, JP;(2) Faculty of Agriculture, Ibaraki University, Ami-machi, Ibaraki 300-3, Japan, JP
Abstract:The human lysozyme gene, which is assembled by the stepwise ligation of chemically synthesized oligonucleotides, was introduced into tobacco (Nicotiana tabacum cv `SR1') by the Agrobacterium-mediated method. The introduced human lysozyme gene was highly expressed under the control of the cauliflower mosaic virus 35S promoter, and the gene product accumulated in the transgenic tobacco plants. The transgenic tobacco plants showed enhanced resistance against the fungus Erysiphe cichoracearum – both conidia formation and mycelial growth were reduced, and the size of the colony was diminished. Microscopic observation revealed that the transgenic tobacco plants carried the resistant phenotype, analogous to that of the resistant cultivar `Kokubu' which had been selected by conventional breeding. Growth of the phytopathogenic bacterium Pseudomonas syringae pv. tabaci was also strongly retarded in the transgenic tobacco, and the chlorotic halo of the disease symptom was reduced to 17% of that observed in the wild-type tobacco. Thus, the introduction of a human lysozyme gene is an effective approach to protect crops against both fungal and bacterial diseases. Received: 9 September 1996 / Revision received: January 9 1997 / Accepted: 20 February 1997
Keywords:Human lysozyme  Transgenic tobacco plants  Disease resistance
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