Organic nitrogen stimulates caulogenesis from hypocotyl callus of Phyllanthus fraternus |
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Authors: | S Rajasubramaniam P Pardha Saradhi |
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Institution: | (1) Centre for Biosciences, Jamia Millia Islamia, 110 025 New Delhi, India |
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Abstract: | Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10–7, 10–6 and 10–5M). Adventitious shoots differentiated from callus developed from the cut ends of 12.5% of the hypocotyl segments cultured on medium supplemented with 10–6M BAP in combination with 10–6M 2,4-D or 10–6M NAA. Profuse rooting occurred from the hypocotyl explants on medium supplemented with 10–6M BAP + 10–6M NAA. Incorporation of casein hydrolysate in B5 medium along with 10–6M BAP + 10–7M 2,4-D enhanced the frequency of cultures with adventitious shoots upto 68.0%. Glutamine, glutamic acid or proline could partially substitute for the effect of casein hydrolysate. Amongst the hypocotyls from 3–14 d old seedlings, the best caulogenesis was obtained with hypocotyls from 7 d old seedlings both in presence or absence of casein hydrolysate. Best rooting of shoots was achieved on half-strength B5 medium supplemented with 10–6M IBA. After hardening, plantlets were successfully transferred to the soil.Abbreviations BAP
6-benzylaminopurine
- 2,4-D
2, 4-dichlorophenoxyacetic acid
- IBA
indole-3-butyric acid
- NAA
1-naphthaleneacetic acid
- CH
casein hydrolysate
- Arg
L-arginine
- Glu
L-glutamic acid
- Gln
L-glutamine
- Leu
L-leucine
- Lys
L-lysine
- Pro
L-proline |
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