Rice recycling: a simple strategy to improve conidia production in solid-state cultures

Here we studied at a laboratory scale a potential strategy to revalorize the residual rice remaining at the end of a conventional conidia production process in solid-state culture. The conidia production of Trichoderma asperellum Th-T4 (3) and Metarhizium robertsii Xoch-8.1 started with the use of fresh rice (unrecycled rice) as the substrate (cycle one), and continued with the use of recycled rice in successive cycles of conidia production. The rice remaining at the end of the first cycle was reused without any further sterilization or reinoculation. As a result, it was observed that the conidia production and productivity significantly increased in both fungi. Conidia production in T. asperellum Th-T4 (3) increased from 1 × 10⁹ (first cycle) to 2·9 × 10⁹ conidia per gram of initial dry substrate (con⋅gds⁻¹) (second cycle using recycled rice), while in M. robertsii Xoch-8.1, this parameter increased form 5·7 × 10⁸ to 1·4 × 10⁹ con⋅gds⁻¹. Both fungi grew faster and conidiated earlier when recycled rice was used as the substrate, therefore, conidia productivity was also significantly improved. Furthermore, the use of recycled rice did not affect conidia viability. This is the first report about a recycling methodology completely free of extra-processing steps, and useful to increase conidia production and productivity.