Dried gel hybridization in place of Southern hybridization for detection of Listeria monocytogenes DNA fragments |
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Authors: | Rong-Fu Wang Wei-Wen Cao MG Johnson |
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Institution: | Department of Food Science, University of Arkansas, Fayetteville 72703. |
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Abstract: | A simple, sensitive, dried gel DNA hybridization method for detection of Listeria monocytogenes DNA fragments is described. DNA samples were fractionated on an agarose gel. The gel was then denatured in NaOH-NaCl and neutralized in Tris-NaCl. The resulting agarose gel was dried and hybridized with 32P-labelled DNA probe. No transfer to nitrocellulose membranes was used. |
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