Flow cytometric analysis of Lactobacillus plantarum to monitor lag times, cell divisionand injury |
| |
| Authors: | JE Ueckert G Nebe von-Caron AP Bos & PF ter Steeg |
| |
| Institution: | Unilever Research Laboratorium, Vlaardingen, The Netherlands,;Unilever Research Laboratory, Sharnbrook, Bedfordshire, UK |
| |
| Abstract: | Flow cytometry in combination with fluorescent molecular markers 5- (and 6-)carboxyfluorescein succinimidylester (CFSE) and propidium iodide (PI) have been applied todetermine lag times, numbers of cell divisions and injury after mild heat (50°C, 5 min) andnisin treatments (0·1 and 1·0 μg ml−1) of Lactobacillus plantarum. Initial labelling with covalently bound dye CFSE (20 and 100 μg ml−1)allowed determination of lag times and cell proliferation for up to eight generations.Double-labelling with CFSE and PI (5 μg ml−1) provided additional informationabout damage levels and distributions within populations. Subpopulations surviving treatmentcould be identified easily and selectively sorted. |
| |
| Keywords: | |
|
|
