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Control of the growth of Alicyclobacillus acidoterrestris in industrialized orange juice using rosemary essential oil and nisin |
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| Authors: | DAM da Silva MS Fernandes EH Endo ACP Vital EA Britta ME Favero JC Castro PT Matumoto-Pintro BP Dias Filho CV Nakamura M Machinski Junior JMG Mikcha BA de Abreu Filho |
| Institution: | 1. Post-Graduate Program in Food Science, State University of Maringá, Maringá, Paraná, Brazil;2. Department of Pharmacy, State University of Maringá, Maringá, Paraná, Brazil;3. Post-Graduate Program in Biological Sciences, State University of Maringá, Maringá, Paraná, Brazil;4. Department of Pathology, Clinical and Toxicological Analysis, State University of Londrina, Londrina, Paraná, Brazil;5. Post-Graduate Program in Food Science, State University of Maringá, Maringá, Paraná, Brazil
Department of Basic Health Sciences, State University of Maringá, Maringá, Paraná, Brazil;6. Department of Agronomy, State University of Maringá, Maringá, Paraná, Brazil;7. Department of Basic Health Sciences, State University of Maringá, Maringá, Paraná, Brazil;8. Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil |
| Abstract: | The use of rosemary essential oil (RO) and its combination with nisin (RO+N) in preventing the multiplication of Alicyclobacillus acidoterrestris in orange juice was evaluated. The minimum inhibitory and bactericidal concentrations (MIC and MBC) for RO were both 125 μg ml?1 while RO+N displayed a synergistic effect. The use of RO and RO+N at concentrations of 1, 4 and 8× MIC in orange juice for 96 h was evaluated in terms of their sporicidal effectiveness. With regard to the action against A. acidoterrestris spores, RO at 8× MIC was sporostatic, whereas RO+N at 1× MIC was sporicidal. Morphological changes in the structure of the micro-organism after treatment were also observed by microscopy. Furthermore, flow cytometric analysis showed that most cells were damaged or killed after treatment. In general, the antioxidant activity after addition of RO+N decreased with time. The results demonstrate that using the combination of RO and nisin can prevent the A. acidoterrestris growth in orange juice. |
| Keywords: | Alicyclobacillus acidoterrestris antioxidant activity essential oil natural antimicrobials synergism |