The response of denitrifiers in a sandy loam soil affected by a long-term fertilization to organic carbon and nitrate |
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Authors: | M Šimek |
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Institution: | (1) Institute of Soil Biology, Academy of Sciences of the Czech Republic & Faculty of Biological Sciences, University of South Bohemia, 370 05 České Budějovice, Czech Republic |
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Abstract: | The response of denitrifiers to carbon in the form of glucose (Glc-C) and nitrate (NO
3
−
-N) amendments was studied in four differently fertilized plots of sandy-loam soil. Two basically different characteristics
of denitrification activity were determined: (1) potential denitrification measured as nitrous oxide production during 1-d incubation in the presence of acetylene, and (2) denitrifying enzyme activity determined in soil slurries as a N2O production in the presence of acetylene and chloramphenicol during 1 h of incubation. Potential denitrification was strongly
influenced by both Glc-C and NO
3
−
-N amendments in their various combinations, but was also affected by the fertilization practice. The response of denitrifiers
to Glc-C and NO
3
−
-N was generally lower in unfertilized and surprisingly also in highly fertilized soils than in organically and moderately
fertilized soils. Denitrifying enzyme activity was stimulated by the fertilization and was, in contrast to potential denitrification,
the highest in highly fertilized soil. The results indicate that although active denitrifiers were present in a highly fertilized
soil, their ability to develop under optimal conditions was decreased (being similar to that of denitrifiers in unfertilized
soil). This suggests long-term changes in soil microbial community in a highly fertilized soil, presumably connected to changes
in soil chemistry caused by fertilization. |
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Keywords: | |
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