Determination of phenolic compounds and diterpenes in roots of Salvia miltiorrhiza and Salvia przewalskii by two LC–MS tools: Multi-stage and high resolution tandem mass spectrometry with assessment of antioxidant capacity |
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| Institution: | 1. Department of Pharmaceutical Botany and Plant Biotechnology, Poznań University of Medical Sciences, ?w. Marii Magdaleny 14, 61-861 Poznań, Poland;2. Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznań, Poland;3. Department of Pathogen Genetics and Plant Resistance, Metabolomics Team, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland;4. Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Z. Noskowskiego 12/14, 61-704 Poznań, Poland;5. Department of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland;6. Department of Pharmacology, Poznań University of Medical Sciences, Rokietnicka 5a, 60-806 Poznań, Poland;7. Department of Botany, Breeding and Agricultural Technology for Medicinal Plants, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznań, Poland;8. Division of Perinatology and Women’s Diseases, Poznań University of Medical Sciences, Polna 33, 60-535 Poznań, Poland;9. Laboratory of Molecular Biology, Poznań University of Medical Sciences, Polna 33, 60-535 Poznań, Poland;1. Drug Research Program, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, P.O. Box 56, FI-00014, University of Helsinki, Finland;2. Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, P.O. Box 56, FI-00014, University of Helsinki, Finland;1. Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey;2. Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, Wroclaw, Poland;3. Bioinformatics and High Performance Computing Research Group, Universidad Católica San Antonio de Murcia (UCAM), Spain;4. Department of Food and Nutrition Technology, Universidad Católica San Antonio de Murcia (UCAM), Spain;5. Department of Weed Science and Soil Tillage Systems, IUNG-Institute of Soil Science and Plant Cultivation, Wroclaw, Poland;1. Université de Constantine 1, Département de chimie, Laboratoire d’Obtention des Substances Thérapeutiques (LOST), Campus Chaabet-Ersas, 25000 Constantine, Algeria;2. Groupe Isolement et Structure, Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312, UFR de Pharmacie, BP 1039, 51687 Reims, France;3. Service Commun d’Analyses, Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312, Bat. 18 B.P.1039, 51687 Reims Cedex 2, France;1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China;2. Agilent Technologies, 3 Wangjing North Road, Beijing 100102, China;1. Hellenic Agricultural Organization DEMETER, Institute of Plant Breeding and Genetic Resourses- IPB&GR, Department of Medicinal and Aromatic Plants, Thermi, 57001 Thessaloniki, Greece;2. Department of Food Quality and Nutrition Department, IASMA Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010 San Michele all''Adige, (TN), Italy;1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China;2. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, PR China;3. Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, Shenyang 110016, PR China;4. Shaanxi University of Chinese Medicine, Xi’an 712046, PR China |
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| Abstract: | Comparative phytochemical analyses of hydroalcoholic (50% EtOH) extracts from roots of S. miltiorrhiza (SM) and S. przewalskii (SP) were performed using two complementary LC–MS systems: the first system HPLC-DAD-MSn an ion trap mass spectrometer and the second system consisted high resolution MS/MS Orbitrap mass spectrometer. The individual compounds were identified using a previously published approach via comparison of the exact molecular masses, mass spectra and retention times to those of standard compounds, online available databases and literature data. Moreover, the determination of antioxidative activities of extracts by DPPH and FRAP methods was carried out. Analysis allowed to identify 39 chemical compounds in extracts from both species. Extract from root of SP differs from SM in the presence of several metabolites such as: przewalskinic acid and their derivatives, przewaquinone C, przewaquinonate A, glycosides of rosmarinic acid, methyltanshinonate, whereas tanshinones, salvianolic acids and lithospermic acids occurred in both species. Moreover, it was shown that hydroalcoholic extract from roots of SM exerted stronger antioxidant properties in a FRAP test (max. 323.92 μM Fe2+/L) and in DPPH test (max. 78.64 nM TE) in comparison with SP extract. |
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| Keywords: | Salvia Root 50% ethanol extract HPLC UPLC Antiradical scavenger activity |
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