Streptomyces-derived actinomycin D inhibits biofilm formation by Staphylococcus aureus and its hemolytic activity |
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Authors: | Jin-Hyung Lee Yong-Guy Kim Kayeon Lee Chang-Jin Kim Dong-Jin Park Yoonjung Ju |
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Institution: | 1. School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea;2. Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea |
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Abstract: | Staphylococcus aureus is a versatile human pathogen that produces diverse virulence factors, and its biofilm cells are difficult to eradicate due to their inherent ability to tolerate antibiotics. The anti-biofilm activities of the spent media of 252 diverse endophytic microorganisms were investigated using three S. aureus strains. An attempt was made to identify anti-biofilm compounds in active spent media and to assess their anti-hemolytic activities and hydrophobicities in order to investigate action mechanisms. Unlike other antibiotics, actinomycin D (0.5 μg ml?1) from Streptomyces parvulus significantly inhibited biofilm formation by all three S. aureus strains. Actinomycin D inhibited slime production in S. aureus and it inhibited hemolysis by S. aureus and caused S. aureus cells to become less hydrophobic, thus supporting its anti-biofilm effect. In addition, surface coatings containing actinomycin D prevented S. aureus biofilm formation on glass surfaces. Given these results, FDA-approved actinomycin D warrants further attention as a potential antivirulence agent against S. aureus infections. |
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Keywords: | Actinomycin D biofilm hemolysis hydrophobicity Staphylococcus aureus |
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