Impact of Pulsed Nd:YAG Laser Irradiation on the Growth and Mortality of the Biofilm Forming Marine Bacterium Pseudoalteromonas carrageenovora |
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Authors: | Kanavillil Nandakumar Hideki Obika Tatsuya Shinozaki Toshihiko Ooie Akihiro Utsumi Tetsuo Yano |
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Institution: | 1. Marine Eco-materials Research Group, National Institute of Advanced Industrial Science and Technology (AIST) , Marine Resources and Environment Research Institute , 2217-14 Hayashi-cho, Takamatsu, Kagawa, 761-0395, Japan;2. Marine Eco-materials Research Group, National Institute of Advanced Industrial Science and Technology (AIST) , Marine Resources and Environment Research Institute , 2217-14 Hayashi-cho, Takamatsu, Kagawa, 761-0395, Japan |
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Abstract: | The impact of pulsed laser irradiation on the marine biofilm forming bacterium Pseudoalteromonas carrageenovora was investigated in the laboratory by monitoring mortality and the post-irradiation growth pattern. The impact of laser irradiation on bacterial mortality increased with the duration of irradiation. Laser irradiation at 532 nm (0.1 J cm m 2 ) for 15 min resulted in a 53% cell mortality immediately after irradiation. However, the impact after a period of 5 h (delayed impact) was more severe. The growth pattern of irradiated samples showed a prolonged lag phase compared to the reference, due to a reduction in total viable counts (TVC) in the irradiated samples. Nucleic acid staining is suggested to be a promising technique for monitoring laser inflicted bacterial mortality. Thus, the results suggest that laser irradiation could be considered as an alternative technique to reduce the number of biofilm forming bacteria and thereby biofilm formation on hard surfaces. |
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Keywords: | Pulsed Laser Irradiation Biofilm Bacteria Mortality Staining Technique Antifouling Technique |
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