| 基于均匀设计法优化三种槭树属植物高效快繁体系 |
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| 引用本文： | 顾地周,;郭玉昕,;高捍东,;孙忠林.基于均匀设计法优化三种槭树属植物高效快繁体系[J].生物数学学报,2009(3):545-550. |
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| 作者姓名： | 顾地周 ;郭玉昕 ;高捍东 ;孙忠林 |
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| 作者单位： | [1]通化师范学院生物系,吉林通化134002; [2]南京林业大学森林资源与环境学院,江苏南京210037; [3]通化市实验中学数学组,吉林通化134001 |
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| 基金项目： | 国家科技部“国家科技攻关计划引导项目”（2005BA741C）;通化师范学院自然科学基金资助项目（200740） |
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| 摘 要： | 以花楷械、青楷械和小楷械的嫩茎段为外植体，应用均匀设计法筛选最适合于嫩茎腋芽生长伸长同时生根的培养基．结果表明，最适合花楷械的培养基为：改良MS＋IAA0．10mg·L^-1＋GAa1．10mg·L^-1，再生率为98％；最适合青楷械的培养基为：改良MS＋IAA0．40mg·L^-1＋IBA0．10mg·L^-1＋NAA0．07mg·L^-1＋GA31．10mg·L^-1，再生率为96％；最适合小楷械的培养基为：改良MS＋IBA0．10mg·L^-1＋GA31．10mg·L^-1，再生率为94％．以再生植株的茎节为材料进行快繁的结果表明，在30-35d的一个培养周期内，增殖倍数达65以上．
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| 关 键 词： | 花楷械 青楷械 小楷械 均匀设计 组织培养 快速繁殖 |
Uniform Design Methods for High Efficient Micropropagation System Optimization of Three Plants of Acer |
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| Institution: | GU Di-zhou GUO Yu-xin GAO Han-dong SUN Zhong-lin (1 Department of Biology, Tonghua Normal University, Tonghua, Tonghua Jilin 134002 China;2 College of Forest Resources and Environment Nanjing Forestry University, Nanjing Jiangsu 210037 China;3 Mathematics Section, Shiyan Middle School of Tonghua City, Tonghua Jilin 134001 China) |
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| Abstract: | Young stems of Acer ukurunduense Trautv. et Mey., Acer tegmentosum Maxim., and Acer komarovii Pojark. were used as explants and suitable medium compositions for axillary bud growing and rooting were screened through Uniform Design experiments. The results showed that MS （modified）＋IAA0.10 mg·L^-1＋GA31.10 mg·L^-1was most suitable for Acer ukurunduense with a regeneration rate of 98%; MS （modified） ＋ IAA0.40 mg·L^-1＋IBA0.10 mg·L^-1＋NAA0.07 mg·L^-1＋GA31.10 mg·L^-1was most suitable for Acer tegmentosum with a regeneration rate of 96%; MS （modified）＋IBA0.10 mg·L^-1＋GA31.10 mg·L^-1was most suitable for Acer kornarovii with a regeneration rate of 94%. Stems each with one node were cut from regenerated shoots and cultured for propagation, and a 65-fold proliferation rate was achieved within 30-35 days. |
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| Keywords: | Acer ukurunduense Trautv et Mey Acer tegmentosum Maxim Acer komarovii Pojark Uniform Design tissue culture micropropagation |
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