Species confirmation of fungal isolates by molecular analysis |
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Authors: | Rupesh Thakur Sardul S Sandhu |
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Institution: | (1) Laboratory of Insect Physiology, Hebrew University, Rehovot Campus, Rehovot, Israel;(2) ARS Biological Integrated Management Research Unit, USDA, Ithaca, NY, USA |
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Abstract: | Traditional taxonomy of hyphomycetes has been based on conidial morphology and development. In order to confirm species level
for the detection and identification of the entomopathogenic fungus, we analysed the species-specific fingerprints to investigate
molecular characteristics within isolates of six species and to resolve morphologically atypical isolates. The extent of fingerprint
profile observed by RAPD was sufficient to confirm the species level of all the isolates. The genetic similarity among morphologically
identified isolates of each species was considerably higher, allowing us to conclude that all the isolates are of same species.
These results establish a molecular framework for further taxonomic, phylogenetic and comparative biological investigations. |
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Keywords: | Beauveria bassiana Entomopathogenic fungi Isaria felina Metarhizium anisopliae Nomuraea rileyi |
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