Prevalence of Very Low Numbers of Potential Pathogenic Isolates of <Emphasis Type="Italic">Yersinia</Emphasis><Emphasis Type="Italic">enterocolitica</Emphasis> and <Emphasis Type="Italic">Yersinia intermedia</Emphasis> in Traditional Fast Foods of India |
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Authors: | K H Divya M C Varadaraj |
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Institution: | (1) Human Resource Development, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, 570 020, India; |
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Abstract: | In this study, an incidence pattern of 1.7% for Yersinia
enterocolitica and 2.5% for Y. intermedia were observed in an analysis of 120 diversified food samples collected from the local market of Mysore, Southern India. Two
native isolates characterized as Y. enterocolitica belonged to biotype 1B and revealed the presence of major virulence related traits such as regulator of virulence, mucoid
Yersinia factor regulator, attachment invasion locus, heat stable enterotoxin, Yersinia type II secretory system and phospholipase A in PCR. Force type neighbor-joining phylograms generated for Y. enterocolitica based on PCR amplicons of rovA and ypl showed 100% homology with two to three strains of Y. enterocolitica and about 75% homology with several strains of Y. pestis. |
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Keywords: | Yersinia enterocolitica Yersinia intermedia PCR detection Virulence determinants |
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