Biochemical and Cytological Studies During Seed Development in Somaclones of Lathyrus sativus |
| |
Authors: | Shalini Singh R P Johari S L Mehta |
| |
Institution: | 1. Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012, India
|
| |
Abstract: | Soluble proteins in six somaclones and the parent at three different stages of development of Lathyrus sativus seeds showed polypeptides of mol wts ranging from 20–165 kD on SDS-PAGE. Immature seeds showed eight distinct esterase bands and two peroxidase bands. Pattern for both the enzymes among somaclones was almost similar. During maturation a new isoform of peroxidase with high mobility appeared in all the somaclones, while one new isoform of intermediate mobility was visible in somaclones Bio L-08 and Bio I-08. Cytological analysis of all the somaclones confirmed the stability of the chromosome count of 2n=14. At metaphase I seven distinct bivalents and at anaphase I and II univalents showing complements of the seven chromosomes were visible. No structural or chromosomal aberration were observed among somaclones. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |