Use of chitin to improve a Beauveria bassiana alginate-pellet formulation |
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Authors: | MacArena Gerding-González Andres France Maria E Sepulveda Jorge Campos |
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Institution: | 1. Facultad de Agronomía , Universidad de Concepción , Chillán , Chile;2. Departamento de Producción Vegetal , Instituto de Investigaciones Agropecuarias (INIA) , Chillán , Chile |
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Abstract: | A study was undertaken to evaluate optimum concentrations of chitin in sodium alginate pellet formulations to enhance conidia production. Chitin concentrations tested were 0, 0.5, 1, 2, 3 and 4% (w/v), with (2%, w/v) or without wheat bran. The different chitin-wheat bran pellet combinations were prepared with Beauveria bassiana isolate Qu-B306 at 108 conidia mL-1. After 21 days of incubation in a humid chamber at 28°C, conidia production was assessed. Improvements up to three times the initial conidia number were achieved using 2% chitin and 2% wheat bran. Higher levels of chitin decreased the number of conidia per pellet. For all chitin concentrations, conidia number increased with the addition of wheat bran (P≤0.05). Contamination by saprophytic fungi was reduced by the incorporation of chitin in the pellet formulation. |
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Keywords: | Entomopathogenic fungi Beauveria alginate formulation encapsulation mycoinsecticide |
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