Specific oxygen uptake of the entomopathogenic nematode,Steinernema carpocapsae CABA01, in submerged culture |
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| Authors: | Norberto Chavarría-Hernández Nalleli-Concepción Pérez-Pérez Juan-Carlos Chavarría-Hernández Luis-Felipe Barahona-Pérez Adriana-Inés Rodríguez-Hernández |
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| Institution: | 1. Cuerpo Académico de Biotecnología Agroalimentaria, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Tulancingo, Mexico;2. Unidad de Energía Renovable, Centro de Investigación Científica de Yucatán, Mérida, Mexico |
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| Abstract: | The specific oxygen uptake rate (qO2) of stages of the entomopathogenic nematode (EPN) Steinernema carpocapsae CABA01 in liquid culture was measured. Nematodes were grown into previously pasteurised culture broths of their symbiotic bacterium, Xenorhabdus nematophila, in orbitally agitated flask cultures (VL = 125 mL) at N = 150 rpm and T = 25°C. The basal medium contained 3% (w/v) soy trypticase broth and 0.5% (w/v) yeast extract. The EPNs developed from the egg stage to the adult stage exhibiting qO2 values of 1.92, 5.48, 0.48, 0.28 and 0.0014 10?1 mmolO2/(gnematode-wet base h)] for the egg-Juvenile 1 (J1), J2, J3, J4 and the adult stages, respectively. |
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| Keywords: | bioinsecticides Steinernema carpocapsae entomopathogenic nematodes liquid culture specific oxygen demands bioreactors |
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